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densitometry analysis  (Bio-Rad)


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    Bio-Rad densitometry analysis
    Simultaneous loss of ciliary ARL13B and PC1 rescues fibrosis and injury caused by loss of PC1 alone, as indicated by morphological ( a ), molecular ( b, c ), and cellular ( d, e ) analysis. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and <t>densitometry</t> analysis of the myofibroblast marker α-smooth muscle actin (α-SMA) in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 5-6). Scale bars are 200 µm (a) and 50 µm (e). Genotypes in b-e correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.
    Densitometry Analysis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 35737 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/densitometry+analysis/Image+Lab+Software/bio_rxiv__2025__11__20__689124-79-0-9
    Average 99 stars, based on 35737 article reviews
    densitometry analysis - by Bioz Stars, 2026-11
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    Images

    1) Product Images from "Ciliary ARLs drive renal cystogenesis"

    Article Title: Ciliary ARLs drive renal cystogenesis

    Journal: bioRxiv

    doi: 10.1101/2025.11.20.689124

    Simultaneous loss of ciliary ARL13B and PC1 rescues fibrosis and injury caused by loss of PC1 alone, as indicated by morphological ( a ), molecular ( b, c ), and cellular ( d, e ) analysis. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-smooth muscle actin (α-SMA) in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 5-6). Scale bars are 200 µm (a) and 50 µm (e). Genotypes in b-e correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.
    Figure Legend Snippet: Simultaneous loss of ciliary ARL13B and PC1 rescues fibrosis and injury caused by loss of PC1 alone, as indicated by morphological ( a ), molecular ( b, c ), and cellular ( d, e ) analysis. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-smooth muscle actin (α-SMA) in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 5-6). Scale bars are 200 µm (a) and 50 µm (e). Genotypes in b-e correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Techniques Used: Staining, Western Blot, Marker, Control

    ( a ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. (b, c) Densitometry analysis of ( b ) β-catenin and ( c ) cyclin D1 ( n = 3). Genotypes in b, c correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.
    Figure Legend Snippet: ( a ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. (b, c) Densitometry analysis of ( b ) β-catenin and ( c ) cyclin D1 ( n = 3). Genotypes in b, c correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Techniques Used: Western Blot, Control

    Morphological, molecular, and cellular analysis reveal that ARL13B R79Q rescues fibrosis, injury, and Wnt signaling caused by loss of PC1. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-SMA in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 6). ( f ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. ( g , h ) Densitometry analysis of ( g ) β-catenin and ( h ) cyclin D1 ( n = 3). Scale bars are 200 µm ( a ) and 50 µm ( e ). Genotypes in b-h correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.
    Figure Legend Snippet: Morphological, molecular, and cellular analysis reveal that ARL13B R79Q rescues fibrosis, injury, and Wnt signaling caused by loss of PC1. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-SMA in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 6). ( f ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. ( g , h ) Densitometry analysis of ( g ) β-catenin and ( h ) cyclin D1 ( n = 3). Scale bars are 200 µm ( a ) and 50 µm ( e ). Genotypes in b-h correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Techniques Used: Staining, Western Blot, Marker, Control

    Related Articles

    Software:

    Article Title: Ciliary ARLs drive renal cystogenesis
    Article Snippet: Membranes were washed with TBS-T and imaged on an Azure Biosystems imager. .. Densitometry analysis was performed with ImageLab software version 6.1 (Bio-Rad). ..

    Article Title: Accelerated vaccine process development by orthogonal protein characterization
    Article Snippet: .. The gel was stained using Instant Blue (AbCam Cat # ab119211) for > 1 h then destained in water for > 2 h. Densitometry analysis was performed on a Bio-Rad GS-900 calibrated densitometer with Image Lab software. ..

    Article Title: Anti-tumor effects of a novel cell penetrating peptide-based therapeutic approach to target Lactate Dehydrogenase C (LDHC) in triple negative breast cancer
    Article Snippet: Next, membranes were washed, incubated with horseradish Peroxidase (HRP)-linked rabbit secondary antibody (Cell signaling, #7074, 1:5,000) for 1 h at room temperature, and proteins were detected by ECL Plus (Thermo Fisher Scientific, #32209) using the ChemiDoc XRS+ Imaging system (Bio-Rad). .. Images acquisition and densitometry analysis were performed using the IMAGE LAB software v6.1 (Bio-Rad). ..

    Article Title: Cigarette Smoke and Decreased DNA Repair by Xeroderma Pigmentosum Group C Use a Double Hit Mechanism for Epithelial Cell Lung Carcinogenesis
    Article Snippet: .. Densitometry analysis was performed using Image Lab Software from Bio-Rad. ..

    Article Title: Accelerated vaccine process development by orthogonal protein characterization.
    Article Snippet: .. The gel was stained using Instant Blue (AbCam Cat # ab119211) for > 1 h then destained in water for > 2 h. Densitometry analysis was performed on a Bio-Rad GS-900 calibrated densitometer with Image Lab software. ..

    Staining:

    Article Title: Accelerated vaccine process development by orthogonal protein characterization
    Article Snippet: .. The gel was stained using Instant Blue (AbCam Cat # ab119211) for > 1 h then destained in water for > 2 h. Densitometry analysis was performed on a Bio-Rad GS-900 calibrated densitometer with Image Lab software. ..

    Article Title: Accelerated vaccine process development by orthogonal protein characterization.
    Article Snippet: .. The gel was stained using Instant Blue (AbCam Cat # ab119211) for > 1 h then destained in water for > 2 h. Densitometry analysis was performed on a Bio-Rad GS-900 calibrated densitometer with Image Lab software. ..

    Recombinant:

    Article Title: Biochemical Properties and Physiological Roles of Tyrosine Aminotransferases in Olive (Olea europaea L.)
    Article Snippet: Finally, protein extracts were concentrated using a Vivaspin centrifugal concentrator (MWCO 50 kDa, Merck, Darmstadt, Germany), and stored at 4 ◦C in storage buffer (50 mM KPi, pH 7.5, 200 μM PLP, 10% glycerol). .. The purity of the recombinant proteins was evaluated by SDS-PAGE and densitometry analysis (ChemiDoc Imaging System, BioRad, Hercules, CA, USA), and the protein concentration was determined by the Bradford assay [26] with bovine serum albumin (BSA) as standard. ..

    SDS Page:

    Article Title: Biochemical Properties and Physiological Roles of Tyrosine Aminotransferases in Olive (Olea europaea L.)
    Article Snippet: Finally, protein extracts were concentrated using a Vivaspin centrifugal concentrator (MWCO 50 kDa, Merck, Darmstadt, Germany), and stored at 4 ◦C in storage buffer (50 mM KPi, pH 7.5, 200 μM PLP, 10% glycerol). .. The purity of the recombinant proteins was evaluated by SDS-PAGE and densitometry analysis (ChemiDoc Imaging System, BioRad, Hercules, CA, USA), and the protein concentration was determined by the Bradford assay [26] with bovine serum albumin (BSA) as standard. ..

    Imaging:

    Article Title: Biochemical Properties and Physiological Roles of Tyrosine Aminotransferases in Olive (Olea europaea L.)
    Article Snippet: Finally, protein extracts were concentrated using a Vivaspin centrifugal concentrator (MWCO 50 kDa, Merck, Darmstadt, Germany), and stored at 4 ◦C in storage buffer (50 mM KPi, pH 7.5, 200 μM PLP, 10% glycerol). .. The purity of the recombinant proteins was evaluated by SDS-PAGE and densitometry analysis (ChemiDoc Imaging System, BioRad, Hercules, CA, USA), and the protein concentration was determined by the Bradford assay [26] with bovine serum albumin (BSA) as standard. ..

    Protein Concentration:

    Article Title: Biochemical Properties and Physiological Roles of Tyrosine Aminotransferases in Olive (Olea europaea L.)
    Article Snippet: Finally, protein extracts were concentrated using a Vivaspin centrifugal concentrator (MWCO 50 kDa, Merck, Darmstadt, Germany), and stored at 4 ◦C in storage buffer (50 mM KPi, pH 7.5, 200 μM PLP, 10% glycerol). .. The purity of the recombinant proteins was evaluated by SDS-PAGE and densitometry analysis (ChemiDoc Imaging System, BioRad, Hercules, CA, USA), and the protein concentration was determined by the Bradford assay [26] with bovine serum albumin (BSA) as standard. ..

    Bradford Assay:

    Article Title: Biochemical Properties and Physiological Roles of Tyrosine Aminotransferases in Olive (Olea europaea L.)
    Article Snippet: Finally, protein extracts were concentrated using a Vivaspin centrifugal concentrator (MWCO 50 kDa, Merck, Darmstadt, Germany), and stored at 4 ◦C in storage buffer (50 mM KPi, pH 7.5, 200 μM PLP, 10% glycerol). .. The purity of the recombinant proteins was evaluated by SDS-PAGE and densitometry analysis (ChemiDoc Imaging System, BioRad, Hercules, CA, USA), and the protein concentration was determined by the Bradford assay [26] with bovine serum albumin (BSA) as standard. ..



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    Image Search Results


    Simultaneous loss of ciliary ARL13B and PC1 rescues fibrosis and injury caused by loss of PC1 alone, as indicated by morphological ( a ), molecular ( b, c ), and cellular ( d, e ) analysis. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-smooth muscle actin (α-SMA) in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 5-6). Scale bars are 200 µm (a) and 50 µm (e). Genotypes in b-e correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Journal: bioRxiv

    Article Title: Ciliary ARLs drive renal cystogenesis

    doi: 10.1101/2025.11.20.689124

    Figure Lengend Snippet: Simultaneous loss of ciliary ARL13B and PC1 rescues fibrosis and injury caused by loss of PC1 alone, as indicated by morphological ( a ), molecular ( b, c ), and cellular ( d, e ) analysis. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-smooth muscle actin (α-SMA) in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 5-6). Scale bars are 200 µm (a) and 50 µm (e). Genotypes in b-e correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Article Snippet: Densitometry analysis was performed with ImageLab software version 6.1 (Bio-Rad).

    Techniques: Staining, Western Blot, Marker, Control

    ( a ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. (b, c) Densitometry analysis of ( b ) β-catenin and ( c ) cyclin D1 ( n = 3). Genotypes in b, c correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Journal: bioRxiv

    Article Title: Ciliary ARLs drive renal cystogenesis

    doi: 10.1101/2025.11.20.689124

    Figure Lengend Snippet: ( a ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. (b, c) Densitometry analysis of ( b ) β-catenin and ( c ) cyclin D1 ( n = 3). Genotypes in b, c correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Article Snippet: Densitometry analysis was performed with ImageLab software version 6.1 (Bio-Rad).

    Techniques: Western Blot, Control

    Morphological, molecular, and cellular analysis reveal that ARL13B R79Q rescues fibrosis, injury, and Wnt signaling caused by loss of PC1. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-SMA in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 6). ( f ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. ( g , h ) Densitometry analysis of ( g ) β-catenin and ( h ) cyclin D1 ( n = 3). Scale bars are 200 µm ( a ) and 50 µm ( e ). Genotypes in b-h correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Journal: bioRxiv

    Article Title: Ciliary ARLs drive renal cystogenesis

    doi: 10.1101/2025.11.20.689124

    Figure Lengend Snippet: Morphological, molecular, and cellular analysis reveal that ARL13B R79Q rescues fibrosis, injury, and Wnt signaling caused by loss of PC1. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-SMA in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 6). ( f ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. ( g , h ) Densitometry analysis of ( g ) β-catenin and ( h ) cyclin D1 ( n = 3). Scale bars are 200 µm ( a ) and 50 µm ( e ). Genotypes in b-h correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Article Snippet: Densitometry analysis was performed with ImageLab software version 6.1 (Bio-Rad).

    Techniques: Staining, Western Blot, Marker, Control